They appear to be directly transmitted through the soil. Bait melon plants grown in the contaminated soil were colonized by both olpidium species and by m. This article is from bmc evolutionary biology, volume 11. General information about olpidium bornovanus olpibo. Olpidium bornovanus olpibooverview eppo global database. It has been observed in several countries of the americas, africa, asia, and europe. Host specificity and nomenclature of olpidium bornovanus olpidium radicale and comparisons to olpidium brassicae. Pdf a multigene phylogeny of olpidium and its implications for. Melon necrotic spot virus mnsv can cause significant economic losses in melon c. A stepwise detection system of obligate parasitic fungi in. Virus transmission by hostspecific strains of olpidium.
Olpidium bornovanus olpibodocuments eppo global database. Our laboratory has developed a novel multiplex pcr assay for the rapid and simultaneous detection and differentiation of three olpidium spp. Some samples tested in this study were found to be dually infected with o. A multigene phylogeny of olpidium and its implications for early fungal evolution satoshi sekimoto1,3,dann rochon2, jennifer e long1,4, jaclyn m dee1 and mary l berbee1. We do not use these to store personal information about you. Resistance to melon necrotic spot virus in cucumis melo l. To our knowledge, no previous studies have reported natural mixed infections of olpidium. For example, olpidium brassicae transmits bigvein virus and big vein associated varicosavirus among lettuce. Accurate taxonomic knowledge is the foundation for further discussions and studies of pathogenicity and ecological role. Olpidium brassicae is commonly present in roots of field crops, especially in the members of brassicaceae, including canola. Multiplex pcr assay for the simultaneous detection and. First report of olpidium virulentus on lettuce in turkey springerlink.
Olpidium bornovanus, a unicellular fungus, is an obligate parasite of plants that reproduces with flagellated. We developed a quantitative detection system for olpidium bornovanus. A multigene phylogeny of olpidium and its implications for. For the control of viral diseases caused by olpidium species, it is important to quantitatively detect the presence of such fungi in field soil.
Download fulltext pdf download fulltext pdf a multigene phylogeny of olpidium and its implications for early fungal evolution article pdf available in bmc evolutionary biology 111. We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services. Relationships between the pathogen olpidium virulentus and viruses associated with lettuce bigvein disease. In particular, we clarify the differences between o. Relationship between the lettuce bigvein virus and its vector, olpidium brassicae. Results document, for the first time, the role of soil moisture on a unique, tripartite, hostspecific rhizosphere interaction i. Cnv is transmitted by the chytrid fungus olpidium bornovanus. A multigene phylogeny of olpidium and its implications for early. Taxonomy and pathogenicity of olpidium brassicae and its allied. Some species may be transmitted through the seed at a very. Although bacteria or actinomycetes were heretofore suspected as the germinationinducing microbes, our data demonstrate that olpidium bornovanus.
Virus transmission by hostspecific strains of olpidium bornovanus. Effect of soil matric water potentials on germination of. A multigene phylogeny of olpidium and its implications for early fungal evolution. This virus attacks crops of cucumber cucumis sativus l. Download fulltext pdf download fulltext pdf first report of olpidium bornovanus and o. Studies on melon necrotic spot virus disease of cucumber and on the control of the fungus vector olpidium radicale authors. Continuing to use this website means you agree to our use of cookies.
Pdf first report of olpidium virulentus on lettuce in turkey. The in vitro virusvector relationship is found between the isometric viruses of the tombusviridae and two olpidium. Ishs xxviii international horticultural congress on science and horticulture for people ihc2010. The protruding domain of the coat protein of melon. A few olpidium species are recognized as fungal vectors of plant viruses. Olpidium bornovanus and melon necrotic spot virus mnsv in. Olpidium bornovanus is the first vector reported for csbv and is confirmed as a vector of sqnv. Documents about olpidium bornovanus olpibo this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. In this thesis, the molecular biology of the interaction between cucumber necrosis tombusvirus cnv and zoospores of its fungal vector, olpidium bornovanus, was examined. Olpidium bornovanusmediated germination of ascospores of. Relationship between the lettuce bigvein virus and its vector. This observation suggested that germination is mediated by one or more heatsensitive members of the soil microflora.
Pdf taxonomy and pathogenicity of olpidium brassicae and its. Molecular characterization of the interaction between. A cucumber necrosis virus variant deficient in fungal. Olpidium virulentus olpidium brassicae, a unicellular fungus parasitizing. Olpidium is a fungal genus in the family olpidiaceae. Involvement of the cucumber necrosis virus coat protein in. International symposium on plant protection influence of grafted watermelon plant. For example, olpidium brassicae transmits bigvein virus and bigvein associated varicosavirus among lettuce. The melon necrotic spot virus is the cause of a serious disorder in greenhouse melon crops. Olpidium bornovanus, a unicellular fungus, is an obligate parasite of plants that reproduces with flagellated, swimming zoospores. Olpidium brassicae is a common fungal parasite of the epidermal cells of plant roots, especially plants in the family brassicaceae formerly cruciferae.
Specifically, colonization of cantaloupe roots by zoospores of o. Analysis of chimeric viruses constructed by switching the coat protein cp gene between the two strains unveiled the involvement of the cp in the attachment of mnsv to zoospores of a compatible isolate of o. Molecular aspects of plant virus transmission by olpidium and. Thus, the combination olpidium bornovanus sahtiyanci karling is accepted.
Association of olpidium bornovanus and melon necrotic spot virus with vine decline of melon in guatemala. Olpidiomycota, rozellomycota and zoopagomycota pdf. Presentation mode open print download current view. Taxonomy and pathogenicity of olpidium brassicae and its. Watermelon production with rootstocks in soils infested. Aleandri mp, martignoni d, reda r, alfarofernandez a, font mi, armengol j chilosi g 2017 involvement of olpidium bornovanus and o. One of the specific amplicons of the expected size 579 nt. Melon necrotic spot virus mnsv is a virus that belongs to the genus carmovirus of the family tombusviridae. Phyto86n12 1294 american phytopathological society. From a common ancestor with animals, the earliest fungi inherited flagellated. Two experiments for watermelon and two experiments with melon were carried out in soils infected with fusarium oxysporum f. The vector also formed the basis for the most sensitive assays of seed borne virus, enzymelinked immunosorbent assay elisa.
Thirty soil borne viruses or virus like agents are transmitted by five species of fungal vectors i. Finding of lettuce big vein virus in czechoslovakia. Topology tests rejected monophyly of the olpidium species with any other clades of flagellated fungi. To our knowledge, no previous studies have reported natural mixed infections of olpidium spp. Department of energy joint genome institute, a doe office of science user facility, is supported by the office of science of the u. Host specificity and nomenclature of olpidium bornovanus.
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